Part:BBa_K2751002:Design

pET32a-FPFSkel1-YPet-Ubc9

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 5167
  Illegal PstI site found at 1140
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 5212
  Illegal PstI site found at 1140
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 5701
  Illegal XhoI site found at 6430
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 5167
  Illegal PstI site found at 1140
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 5167
  Illegal PstI site found at 1140
  Illegal NgoMIV site found at 137
  Illegal NgoMIV site found at 3317
  Illegal NgoMIV site found at 3477
  Illegal NgoMIV site found at 5065
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1314
  Illegal BsaI.rc site found at 6353
  Illegal SapI.rc site found at 2396